Mammary Gland Whole Mount Round Robin Summary

In order to improve reliability and standardization of mammary gland whole mount assessments, directors and staff of seven laboratories from around the world (see attached) participated in a round robin evaluation of a set of mammary gland whole mounts. These preparations were from the 4 and 5 mammary glands of mice and rats treated in utero with vehicle and 2 different chemical treatments. Whole mounts were prepared from control and exposed mammary glands of female offspring at post-natal days 4, 21, and 45. The above-referred scientists, along with six other experts acting as observers, met to review their cumulative assessments and generate standardized guidelines for conducting mammary whole mount assessments. These methods and conclusions will be forthcoming as a scientific publication.